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Highly Efficient Intramolecular Electrochemiluminescence Energy Transfer for Ultrasensitive Bioanalysis of Aflatoxin M1
Author(s) -
Liu JiaLi,
Zhao Min,
Zhuo Ying,
Chai YaQin,
Yuan Ruo
Publication year - 2017
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201604411
Subject(s) - bioanalysis , electrochemiluminescence , aflatoxin , intramolecular force , energy transfer , nanotechnology , chemistry , materials science , chromatography , food science , stereochemistry , detection limit , molecular physics
The intermolecular electrochemiluminescence resonance energy transfer (ECL‐RET) between luminol and Ru(bpy) 3 2+ was studied extensively to achieve the sensitive bioanalysis owing to the perfect spectral overlap of the donor and acceptor, but it still suffers from the challenging issue of low energy‐transfer efficiency. The intramolecular ECL‐RET towards the novel ECL compound containing the donor of luminol and the acceptor of Ru(bpy) 2 (mcpbpy) 2+ (Lum‐Ru) was designed and investigated. With the high‐efficient ECL‐RET in one molecule, the highly intense ECL signal of Lum‐Ru was obtained owing to the short path of energy transmission and less energy loss between luminol and Ru(bpy) 2 (mcpbpy) 2+ . Lum‐Ru was further applied to construct a signal‐off electrochemiluminescence (ECL) aptasensor for ultrasensitive detection of a harsh carcinogen of Aflatoxin M1 (AFM1). This sensing platform also provides a significant boost for the trace detection of other biomolecules in clinical analysis.