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The Lipid A from Rhodopseudomonas palustris Strain BisA53 LPS Possesses a Unique Structure and Low Immunostimulant Properties
Author(s) -
Di Lorenzo Flaviana,
Palmigiano Angelo,
AlbitarNehme Sami,
Sturiale Luisa,
Duda Katarzyna A.,
Gully Djamel,
Lanzetta Rosa,
Giraud Eric,
Garozzo Domenico,
Bernardini Maria Lina,
Molinaro Antonio,
Silipo Alba
Publication year - 2017
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201604379
Subject(s) - immunostimulant , lipid a , rhodopseudomonas palustris , lipopolysaccharide , biochemistry , chemistry , strain (injury) , tetrasaccharide , bacteria , biology , stereochemistry , polysaccharide , immune system , immunology , genetics , anatomy
The search for novel lipid A analogues from any biological source that can act as antagonists, displaying inhibitory activity towards the production of pro‐inflammatory cytokines, or as immunomodulators in mammals, is a very topical issue. To this aim, the structure and immunological properties of the lipopolysaccharide lipid A from the purple nonsulfur bacterium Rhodopseudomonas palustris strain BisA53 have been determined. This lipid A displays a unique structural feature, with a non‐phosphorylated skeleton made up of the tetrasaccharide Man p ‐α‐(1→4)‐Glc p N3N‐β‐1→6‐Glc p N3N‐α‐(1→1)‐α‐Gal p A, and four primary amide‐linked 14:0(3‐OH) and, as secondary O ‐acyl substituents, a 16:0 and the very long‐chain fatty acid 26:0(25‐OAc), appended on the Glc p N3N units. This lipid A architecture is definitely rare, so far identified only in the genus Bradyrhizobium . Immunological tests on both murine bone‐marrow‐derived and human monocyte‐derived macrophages revealed an extremely low immunostimulant capability of this LPS lipid A.