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Filterable Agents for Hyperpolarization of Water, Metabolites, and Proteins
Author(s) -
Vuichoud Basile,
Bornet Aurélien,
de Nanteuil Florian,
Milani Jonas,
Canet Estel,
Ji Xiao,
Miéville Pascal,
Weber Emmanuelle,
Kurzbach Dennis,
Flamm Andrea,
Konrat Robert,
Gossert Alvar D.,
Jannin Sami,
Bodenhausen Geoffrey
Publication year - 2016
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201602506
Subject(s) - hyperpolarization (physics) , chemistry , dissolution , polarization (electrochemistry) , nitroxide mediated radical polymerization , deuterium , polymer , nuclear overhauser effect , radical , nuclear magnetic resonance spectroscopy , organic chemistry , monomer , physics , radical polymerization , quantum mechanics
Hyperpolarization is generated by dissolution dynamic nuclear polarization (d‐DNP) using a polymer‐based polarizing agent dubbed FLAP (filterable labeled agents for polarization). It consists of a thermo‐responsive poly( N ‐isopropylacrylamide), also known as p NiPAM‐COOH, labeled with nitroxide radicals. The polymer powder is impregnated with an arbitrary solution of interest and frozen as is. Dissolution is followed by a simple filtration, leading to hyperpolarized solutions free from any contaminants. We demonstrated the use of FLAP to hyperpolarize partially deuterated water up to P ( 1 H)=6 % with a long relaxation T 1 >36 s characteristic of high purity. Water hyperpolarization can be transferred to drugs, metabolites, or proteins that are waiting in an NMR spectrometer, either by exchange of labile protons or through intermolecular Overhauser effects. We also show that FLAPs are suitable polarizing agents for 13 C‐labeled metabolites such as pyruvate, acetate, and alanine.