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Surface Enhanced Raman Scattering and Gated Materials for Sensing Applications: The Ultrasensitive Detection of Mycoplasma and Cocaine
Author(s) -
Oroval Mar,
CoronadoPuchau Marc,
Langer Judith,
SanzOrtiz Marta Norah,
Ribes Ángela,
Aznar Elena,
Coll Carmen,
Marcos María Dolores,
Sancenón Félix,
LizMarzán Luis M.,
MartínezMáñez Ramón
Publication year - 2016
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201602457
Subject(s) - raman scattering , detection limit , genomic dna , analyte , dna , mesoporous silica , aptamer , raman spectroscopy , materials science , colloidal gold , bioanalysis , nanotechnology , nanoparticle , chemistry , chromatography , mesoporous material , microbiology and biotechnology , optics , biochemistry , biology , physics , catalysis
Abstract We present herein a novel combination of gated mesoporous silica nanoparticles (MSNs) and surface‐enhanced Raman scattering (SERS) for sensing applications. As a proof‐of‐concept, we show the design of a system comprising MSNs loaded with crystal violet (CV), a molecule with high Raman cross section acting as SERS reporter, and capped with either a suitable DNA sequence for the detection of Mycoplasma genomic DNA or with an aptamer that selectively coordinates cocaine. In both cases the presence of the corresponding target analyte in solution (i.e., genomic DNA or cocaine) resulted in the release of CV. CV delivery was detected by SERS upon adsorption on gold nanotriangles (AuNTs), which display an efficient electromagnetic field enhancement and a high colloidal stability. By using this novel procedure a limit of detection of at least 30 copies DNA per μL was determined for the detection of Mycoplasma genomic DNA, whereas cocaine was detected at concentrations as low as 10 n m .