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Preferential 5‐Methylcytosine Oxidation in the Linker Region of Reconstituted Positioned Nucleosomes by Tet1 Protein
Author(s) -
Kizaki Seiichiro,
Zou Tingting,
Li Yue,
Han YongWoon,
Suzuki Yuki,
Harada Yoshie,
Sugiyama Hiroshi
Publication year - 2016
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201602435
Subject(s) - nucleosome , 5 methylcytosine , linker , histone octamer , dna demethylation , chemistry , dna , histone , demethylation , cpg site , linker dna , chromatin , 5 hydroxymethylcytosine , dna methylation , microbiology and biotechnology , bisulfite , chromatosome , biochemistry , biology , gene , gene expression , computer science , operating system
Tet (ten–eleven translocation) family proteins oxidize 5‐methylcytosine (mC) to 5‐hydroxymethylcytosine (hmC), 5‐formylcytosine (fC), and 5‐carboxycytosine (caC), and are suggested to be involved in the active DNA demethylation pathway. In this study, we reconstituted positioned mononucleosomes using CpG‐methylated 382 bp DNA containing the Widom 601 sequence and recombinant histone octamer, and subjected the nucleosome to treatment with Tet1 protein. The sites of oxidized methylcytosine were identified by bisulfite sequencing. We found that, for the oxidation reaction, Tet1 protein prefers mCs located in the linker region of the nucleosome compared with those located in the core region.