Synthesis of β‐1,4‐Linked Galactan Side‐Chains of Rhamnogalacturonan I

The synthesis of linear‐ and (1→6)‐branched β‐(1→4)‐ d ‐galactans, side‐chains of the pectic polysaccharide rhamnogalacturonan I is described. The strategy relies on iterative couplings of n ‐pentenyl disaccharides followed by a late stage glycosylation of a common hexasaccharide core. Reaction with a covalent linker and immobilization on N ‐hydroxysuccinimide (NHS)‐modified glass surfaces allows the generation of carbohydrate microarrays. The glycan arrays enable the study of protein–carbohydrate interactions in a high‐throughput fashion, demonstrated herein with binding studies of mAbs and a CBM.