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Detection of Sulfatase Enzyme Activity with a CatalyCEST MRI Contrast Agent
Author(s) -
Sinharay Sanhita,
FernándezCuervo Gabriela,
Acfalle Jasmine P.,
Pagel Mark D.
Publication year - 2016
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201600685
Subject(s) - sulfatase , enzyme , chemistry , enzyme assay , biochemistry
A chemical exchange saturation transfer (CEST) MRI contrast agent has been developed that detects sulfatase enzyme activity. The agent produces a CEST signal at δ =5.0 ppm before enzyme activity, and a second CEST signal appears at δ =9.0 ppm after the enzyme cleaves a sulfate group from the agent. The comparison of the two signals improved detection of sulfatase activity.