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Accurate Extraction of Nanometer Distances in Multimers by Pulse EPR
Author(s) -
Valera Silvia,
Ackermann Katrin,
Pliotas Christos,
Huang Hexian,
Naismith James H.,
Bode Bela E.
Publication year - 2016
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201505143
Subject(s) - electron paramagnetic resonance , nanometre , pulsed epr , extraction (chemistry) , pulse (music) , ion , spin (aerodynamics) , membrane , materials science , resonance (particle physics) , electron , nanotechnology , analytical chemistry (journal) , nuclear magnetic resonance , chemistry , physics , spin echo , atomic physics , magnetic resonance imaging , chromatography , optics , medicine , biochemistry , organic chemistry , quantum mechanics , detector , composite material , radiology , thermodynamics
Abstract Pulse electron paramagnetic resonance (EPR) is gaining increasing importance in structural biology. The PELDOR (pulsed electron–electron double resonance) method allows extracting distance information on the nanometer scale. Here, we demonstrate the efficient extraction of distances from multimeric systems such as membrane‐embedded ion channels where data analysis is commonly hindered by multi‐spin effects.