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Electrochemiluminescence of Supramolecular Nanorods and Their Application in the “On–Off–On” Detection of Copper Ions
Author(s) -
Lei YanMei,
Zhao Min,
Wang Ai,
Yu YanQing,
Chai YaQin,
Yuan Ruo,
Zhuo Ying
Publication year - 2016
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201504995
Subject(s) - nanorod , electrochemiluminescence , copper , supramolecular chemistry , ion , nanotechnology , materials science , computer science , chemistry , electrode , molecule , organic chemistry , metallurgy
In this work, an “on–off–on” switch system has been successfully applied through the construction of an electrochemiluminscent biosensor for copper ion (Cu 2+ ) detection based on a new electrochemiluminescence (ECL) emitter of supramolecular nanorods, which was achieved through supramolecular interactions between 3,4,9,10‐perylenetetracarboxylic acid (PTCA) and aniline. The initial “signal‐on” state with strong and stable ECL emission was obtained by use of the supramolecular nanorods with a new signal amplification strategy involving a co‐reaction accelerator. In addition, ECL quencher probes (Fc‐NH 2 /Cu‐Sub/nano‐Au) were fabricated by immobilizing aminoferrocene (Fc‐NH 2 ) on Cu‐substrate strand modified Au nanoparticles. The quencher probes were hybridized with the immobilized Cu‐enzyme strand to form Cu 2+ ‐specific DNAzyme. Similarly, the “signal‐off” state was obtained by the high quenching effect of Fc‐NH 2 on the ECL of the excited‐state PTCA ( 1 PTCA*). As expected, the second “switch‐on” state could achieved by incubating with the target Cu 2+ , owing to the Cu 2+ ‐specific DNAzyme, which was irreversibly cleaved, resulting in the release of the quencher probes from the sensor interface. Herein, on the basis of the ECL intensity changes (Δ I ECL ) before and after incubating with the target Cu 2+ , the prepared Cu 2+ ‐specific DNAzyme‐based biosensor was used for the determination of Cu 2+ concentrations with high sensitivity, excellent selectivity, and good regeneration.