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Targeting Innate Immunity with dsRNA‐Conjugated Mesoporous Silica Nanoparticles Promotes Antitumor Effects on Breast Cancer Cells
Author(s) -
Ultimo Amelia,
Giménez Cristina,
Bartovsky Pavel,
Aznar Elena,
Sancenón Félix,
Marcos M. Dolores,
Amorós Pedro,
Bernardo Ana R.,
MartínezMáñez Ramón,
JiménezLara Ana M.,
Murguía José R.
Publication year - 2016
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201504629
Subject(s) - tlr3 , mesoporous silica , cancer cell , cytotoxic t cell , apoptosis , doxorubicin , innate immune system , chemistry , cytotoxicity , gene silencing , cancer research , mesoporous material , cancer , toll like receptor , receptor , biology , medicine , biochemistry , chemotherapy , in vitro , gene , catalysis
Abstract We describe herein a Toll‐like receptor 3 (TLR3) targeting delivery system based on mesoporous silica nanoparticles capped with the synthetic double stranded RNA polyinosinic–polycytidylic acid (poly(I:C)) for controlled cargo delivery in SK‐BR‐3 breast carcinoma cells. Our results show that poly(I:C)‐conjugated nanoparticles efficiently targeted breast cancer cells due to dsRNA–TLR3 interaction. Such interaction also triggered apoptotic pathways in SK‐BR‐3, significantly decreasing cells viability. Poly(I:C) cytotoxic effect in breast carcinoma cells was enhanced by loading nanoparticles′ mesopores with the anthracyclinic antibiotic doxorubicin, a commonly used chemotherapeutic agent.