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Click Chemistry Mediated Functionalization of Vertical Nanowires for Biological Applications
Author(s) -
Vutti Surendra,
Schoffelen Sanne,
Bolinsson Jessica,
BuchMånson Nina,
Bovet Nicolas,
Nygård Jesper,
Martinez Karen L.,
Meldal Morten
Publication year - 2016
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201504540
Subject(s) - biomolecule , surface modification , biosensor , click chemistry , nanotechnology , covalent bond , chemistry , nanowire , combinatorial chemistry , peptide , cycloaddition , drug delivery , substrate (aquarium) , fluorescence , materials science , biochemistry , catalysis , organic chemistry , physics , quantum mechanics , oceanography , geology
Semiconductor nanowires (NWs) are gaining significant importance in various biological applications, such as biosensing and drug delivery. Efficient and controlled immobilization of biomolecules on the NW surface is crucial for many of these applications. Here, we present for the first time the use of the Cu I ‐catalyzed alkyne–azide cycloaddition and its strain‐promoted variant for the covalent functionalization of vertical NWs with peptides and proteins. The potential of the approach was demonstrated in two complementary applications of measuring enzyme activity and protein binding, which is of general interest for biological studies. The attachment of a peptide substrate provided NW arrays for the detection of protease activity. In addition, green fluorescent protein was immobilized in a site‐specific manner and recognized by antibody binding to demonstrate the proof‐of‐concept for the use of covalently modified NWs for diagnostic purposes using minute amounts of material.