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Installation of a Rigid EDTA‐Like Motif into a Protein α‐Helix for Paramagnetic NMR Spectroscopy with Cobalt(II) Ions
Author(s) -
Swarbrick James D.,
Ung Phuc,
Dennis Matthew L.,
Lee Michael D.,
Chhabra Sandeep,
Graham Bim
Publication year - 2016
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201503139
Subject(s) - paramagnetism , iminodiacetic acid , chemistry , crystallography , cobalt , structural motif , cysteine , metal ions in aqueous solution , ion , metal , helix (gastropod) , nuclear magnetic resonance spectroscopy , stereochemistry , inorganic chemistry , biochemistry , physics , organic chemistry , ecology , quantum mechanics , snail , biology , enzyme
Coupling two copies of an iminodiacetic acid–cysteine hybrid ligand to a pair of cysteine residues positioned in an i , i +4 arrangement within a protein α‐helix leads to generation of an EDTA‐like metal ion‐binding motif. Rigid binding of a Co II ion by this motif produces pseudo‐contact shifts suitable for paramagnetic NMR structural studies.