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Bioorthogonal SERS Nanoprobes for Mulitplex Spectroscopic Detection, Tumor Cell Targeting, and Tissue Imaging
Author(s) -
Wu Junzhou,
Liang Duanwei,
Jin Qingqing,
Liu Jie,
Zheng Meiling,
Duan Xuanming,
Tang Xinjing
Publication year - 2015
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201501942
Subject(s) - bioorthogonal chemistry , aptamer , nanotechnology , raman spectroscopy , raman scattering , multiplex , nucleolin , conjugated system , nanoprobe , materials science , colloidal gold , biophysics , chemistry , nanoparticle , microbiology and biotechnology , cytoplasm , combinatorial chemistry , biochemistry , click chemistry , bioinformatics , biology , optics , physics , composite material , nucleolus , polymer
A surface‐enhanced Raman scattering (SERS) technique shows extraordinary features for a range of biological and biomedical applications. Herein, a series of novel bioorthogonal SERS nanoprobes were constructed with Gold nanoflower (AuNF) and Raman reporters, the signals of which were located in a Raman‐silent region of biological samples. AS1411 aptamer was also co‐conjugated with AuNF through a self‐assembled monolayer coverage strategy. Multiplex SERS imaging using these nanoprobes with three different bioorthogonal small‐molecule Raman reporters is successfully achieved with high multiplexing capacity in a biologically Raman‐silent region. These Raman nanoprobes co‐conjugated with AS1411 showed high affinity for tumor cells with overexpressed nucleolin and can be used for selective tumor cell screening and tissue imaging.