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Iron(II) Supramolecular Helicates Condense Plasmid DNA and Inhibit Vital DNA‐Related Enzymatic Activities
Author(s) -
Malina Jaroslav,
Han Michael J.,
Brabec Viktor
Publication year - 2015
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201501307
Subject(s) - dna , chemistry , dna condensation , topoisomerase , biochemistry , dna polymerase , enzyme , stereochemistry , transfection , gene
The dinuclear iron(II) supramolecular helicates [Fe 2 L 3 ]Cl 4 (L=C 25 H 20 N 4 ) bind to DNA through noncovalent (i.e., hydrogen‐bonding, electrostatic) interactions and exhibit antimicrobial and anticancer effects. In this study, we show that the helicates condense plasmid DNA with a much higher potency than conventional DNA‐condensing agents. Notably, molecules of DNA in the presence of the M enantiomer of [Fe 2 L 3 ]Cl 4 do not form intermolecular aggregates typically formed by other condensing agents, such as spermidine or spermine. The helicates inhibit the activity of several DNA‐processing enzymes, such as RNA polymerase, DNA topoisomerase I, deoxyribonuclease I, and site‐specific restriction endonucleases. However, the results also indicate that the DNA condensation induced by the helicates does not play a crucial role in these inhibition reactions. The mechanisms for the inhibitory effects of [Fe 2 L 3 ]Cl 4 helicates on DNA‐related enzymatic activities have been proposed.