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A Modular Synthesis of Modified Phosphoanhydrides
Author(s) -
Hofer Alexandre,
Cremosnik Gregor S.,
Müller André C.,
Giambruno Roberto,
Trefzer Claudia,
SupertiFurga Giulio,
Bennett Keiryn L.,
Jessen Henning J.
Publication year - 2015
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201500838
Subject(s) - phosphoproteomics , nucleotide , nucleoside , modular design , computational biology , chemistry , combinatorial chemistry , synthetic biology , nucleic acid , biochemistry , nanotechnology , biology , computer science , kinase , materials science , programming language , protein kinase a , protein phosphorylation , gene
Abstract Phosphoanhydrides (P‐anhydrides) are ubiquitously occurring modifications in nature. Nucleotides and their conjugates, for example, are among the most important building blocks and signaling molecules in cell biology. To study and manipulate their biological functions, a diverse range of analogues have been developed. Phosphate‐modified analogues have been successfully applied to study proteins that depend on these abundant cellular building blocks, but very often both the preparation and purification of these molecules are challenging. This study discloses a general access to P‐anhydrides, including different nucleotide probes, that greatly facilitates their preparation and isolation. The convenient and scalable synthesis of, for example, 18 O labeled nucleoside triphosphates holds promise for future applications in phosphoproteomics.