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A Modular Approach To Study Protein Adsorption on Surface Modified Hydroxyapatite
Author(s) -
Ozhukil Kollath Vinayaraj,
Van den Broeck Freya,
Fehér Krisztina,
Martins José C.,
Luyten Jan,
Traina Karl,
Mullens Steven,
Cloots Rudi
Publication year - 2015
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201500223
Subject(s) - adsorption , protein adsorption , thermogravimetric analysis , biomolecule , surface modification , zeta potential , bovine serum albumin , phosphoserine , chemical engineering , chemistry , linker , molecule , materials science , nanotechnology , organic chemistry , nanoparticle , chromatography , serine , computer science , engineering , enzyme , operating system
Biocompatible inorganic nano‐ and microcarriers can be suitable candidates for protein delivery. This study demonstrates facile methods of functionalization by using nanoscale linker molecules to change the protein adsorption capacity of hydroxyapatite (HA) powder. The adsorption capacity of bovine serum albumin as a model protein has been studied with respect to the surface modifications. The selected linker molecules (lysine, arginine, and phosphoserine) can influence the adsorption capacity by changing the electrostatic nature of the HA surface. Qualitative and quantitative analyses of linker‐molecule interactions with the HA surface have been performed by using NMR spectroscopy, zeta‐potential measurements, X‐ray photoelectron spectroscopy, and thermogravimetric analyses. Additionally, correlations to theoretical isotherm models have been calculated with respect to Langmuir and Freundlich isotherms. Lysine and arginine increased the protein adsorption, whereas phosphoserine reduced the protein adsorption. The results show that the adsorption capacity can be controlled with different functionalization, depending on the protein–carrier selections under consideration. The scientific knowledge acquired from this study can be applied in various biotechnological applications that involve biomolecule–inorganic material interfaces.