Guanosine in a Single Stranded Region of Anticodon Stem–Loop tRNA Models is Prone to Oxidatively Generated Damage Resulting in Dehydroguanidinohydantoin and Spiroiminodihydantoin Lesions | Zendy

TomaszewskaAntczak Agnieszka | Zendy; Guga Piotr | Zendy; Nawrot Barbara | Zendy; Pratviel Geneviève | Zendy

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Guanosine in a Single Stranded Region of Anticodon Stem–Loop tRNA Models is Prone to Oxidatively Generated Damage Resulting in Dehydroguanidinohydantoin and Spiroiminodihydantoin Lesions

Author(s) -

TomaszewskaAntczak Agnieszka,

Guga Piotr,

Nawrot Barbara,

Pratviel Geneviève

Publication year - 2015

Publication title -

chemistry – a european journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.687

H-Index - 242

eISSN - 1521-3765

pISSN - 0947-6539

DOI - 10.1002/chem.201406409

Subject(s) - guanine , transfer rna , guanosine , rna , ribose , chemistry , porphyrin , biochemistry , biophysics , biology , nucleotide , gene , enzyme

Oxidation of RNA hairpin models corresponding to anticodon stem–loop (ASL) of transfer RNA led to RNA damage consisting solely of a unique loop guanine oxidation. Manganese porphyrin/oxone treatment of RNA resulted in dehydroguanidinohydantoin ( DGh ; major) and/or spiroiminodihydantoin ( Sp ) lesions. Ribose damage was not observed. This two‐electron transfer oxidation reaction allowed the identification of guanine oxidation products for further study of RNA species carrying a unique lesion at a single G to investigate their biological impact.

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