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Electrospun Highly Ordered Mesoporous Silica–Carbon Composite Nanofibers for Rapid Extraction and Prefractionation of Endogenous Peptides
Author(s) -
Zhu GangTian,
Chen Xi,
He XiaoMei,
Wang Han,
Zhang Zheng,
Feng YuQi
Publication year - 2015
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201406237
Subject(s) - mesoporous material , extraction (chemistry) , composite number , materials science , nanofiber , chromatography , mesoporous silica , elution , adsorption , solid phase extraction , chemical engineering , ion exchange , carbon nanofiber , chemistry , nanotechnology , carbon nanotube , ion , organic chemistry , composite material , catalysis , engineering
A simple method was developed for the preparation of ordered mesoporous silica–carbon composite nanofibers (OMSCFs). The OMSCFs exhibited high carbon content, continuously long fibrous properties, uniform accessible mesopores, and a large surface area. The OMSCFs were also found to have ion‐exchange capacity. On the basis of the size‐exclusion effect of the mesopores and mixed‐mode hydrophobic/ion‐exchange interactions, the OMSCFs were applied for rapid enrichment of endogenous peptides by using a miniaturized solid‐phase extraction format. The adsorption mechanism was studied, and the eluting solution was optimized with standard peptide/protein solutions and protein digests. Employing a successive three‐step elution strategy, followed by LC‐MS/MS analysis, led to excellent performance with this approach in the extraction and prefractionation of peptides from human serum.