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Light‐Triggered Capture and Release of DNA and Proteins by Host–Guest Binding and Electrostatic Interaction
Author(s) -
Moratz Johanna,
Samanta Avik,
Voskuhl Jens,
Mohan Nalluri Siva Krishna,
Ravoo Bart Jan
Publication year - 2015
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201405936
Subject(s) - azobenzene , biomolecule , linker , amphiphile , supramolecular chemistry , chemistry , dna , cyclodextrin , vesicle , dna nanotechnology , biophysics , rational design , small molecule , ternary complex , molecule , nanotechnology , combinatorial chemistry , materials science , membrane , copolymer , biochemistry , polymer , organic chemistry , biology , computer science , operating system , enzyme
The development of an effective and general delivery method that can be applied to a large variety of structurally diverse biomolecules remains a bottleneck in modern drug therapy. Herein, we present a supramolecular system for the dynamic trapping and light‐stimulated release of both DNA and proteins. Self‐assembled ternary complexes act as nanoscale carriers, comprising vesicles of amphiphilic cyclodextrin, the target biomolecules and linker molecules with an azobenzene unit and a charged functionality. The non‐covalent linker binds to the cyclodextrin by host–guest complexation with the azobenzene. Proteins or DNA are then bound to the functionalized vesicles through multivalent electrostatic attraction. The photoresponse of the host–guest complex allows a light‐induced switch from the multivalent state that can bind the biomolecules to the low‐affinity state of the free linker, thereby providing external control over the cargo release. The major advantage of this delivery approach is the wide variety of targets that can be addressed by multivalent electrostatic interaction, which we demonstrate on four types of DNA and six different proteins.