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Disassembly‐Driven Fluorescence Turn‐on of Polymerized Micelles by Reductive Stimuli in Living Cells
Author(s) -
Niko Yosuke,
Arntz Youri,
Mely Yves,
Konishi Genichi,
Klymchenko Andrey S.
Publication year - 2014
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201405040
Subject(s) - fluorescence , nile red , amphiphile , nanotechnology , polymerization , nanoparticle , micelle , nanomaterials , drug delivery , biophysics , materials science , förster resonance energy transfer , biological imaging , linker , small molecule , combinatorial chemistry , chemistry , copolymer , organic chemistry , computer science , polymer , biochemistry , physics , quantum mechanics , aqueous solution , biology , operating system
Stimuli‐response nanoparticles have emerged as powerful tools for imaging and therapeutic applications. Ideally, they should be assembled from biodegradable materials featuring small size and cooperative response to biological stimuli that trigger particle disassembly and release of an active molecule that could be readily monitored in situ. A concept is developed that consists of organic nanoparticles, assembled from fluorescent amphiphiles and polymerized with a redox‐cleavable cross‐linker. We obtained 20 nm nanoparticles bearing self‐quenched Nile Red dye residues, which can disassemble in living cells into highly fluorescent molecular units owing to an external or internal reductive stimulus. The obtained results pave the way to new stimuli‐responsive nanomaterials for applications in background‐free imaging as well as in drug delivery, as the concept can be further extended to other active molecules including drugs and to cross‐linkers cleavable by other biological stimuli.