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Novel Composite Plastics Containing Silver(I) Acylpyrazolonato Additives Display Potent Antimicrobial Activity by Contact
Author(s) -
Marchetti Fabio,
Palmucci Jessica,
Pettinari Claudio,
Pettinari Riccardo,
Condello Francesca,
Ferraro Stefano,
Marangoni Mirko,
Crispini Alessandra,
Scuri Stefania,
Grappasonni Iolanda,
Cocchioni Mario,
Nabissi Massimo,
Chierotti Michele R.,
Gobetto Roberto
Publication year - 2015
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201404812
Subject(s) - antimicrobial , chemistry , contact angle , membrane , nuclear chemistry , cytotoxicity , nanocomposite , composite number , materials science , organic chemistry , composite material , biochemistry , in vitro
New silver(I) acylpyrazolonato derivatives displaying a mononuclear, polynuclear, or ionic nature, as a function of the ancillary azole ligands used in the synthesis, have been fully characterized by thermal analysis, solution NMR spectroscopy, solid‐state IR and NMR spectroscopies, and X‐ray diffraction techniques. These derivatives have been embedded in polyethylene (PE) matrix, and the antimicrobial activity of the composite materials has been tested against three bacterial strains ( E. coli , P. aeruginosa , and S. aureus ): Most of the composites show antimicrobial action comparable to PE embedded with AgNO 3 . Tests by contact and release tests for specific migration of silver from PE composites clearly indicate that, at least in the case of the PE, for composites containing polynuclear silver(I) additives, the antimicrobial action is exerted by contact, without release of silver ions. Moreover, PE composites can be re‐used several times, displaying the same antimicrobial activity. Membrane permeabilization studies and induced reactive oxygen species (ROS) generation tests confirm the disorganization of bacterial cell membranes. The cytotoxic effect, evaluated in CD34 + cells by MTT (3‐(4,5‐dimethylthiazole‐2‐yl)‐2,5‐diphenyltetrazoliumbromide) and CFU (colony forming units) assays, indicates that the PE composites do not induce cytotoxicity in human cells. Studies of ecotoxicity, based on the test of Daphnia magna , confirm tolerability of the PE composites by higher organisms and exclude the release of Ag + ions in sufficient amounts to affect water environment.