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Covalent Bond or Noncovalent Bond: A Supramolecular Strategy for the Construction of Chemically Synthesized Vaccines
Author(s) -
Gao Yue,
Sun ZhanYi,
Huang ZhiHua,
Chen PuGuang,
Chen YongXiang,
Zhao YuFen,
Li YanMei
Publication year - 2014
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201404013
Subject(s) - covalent bond , supramolecular chemistry , non covalent interactions , chemistry , cytotoxicity , antibody dependent cell mediated cytotoxicity , antibody , combinatorial chemistry , biochemistry , hydrogen bond , biology , molecule , immunology , organic chemistry , in vitro
A novel noncovalent strategy to construct chemically synthesized vaccines has been designed to trigger a robust immune response and to dramatically improve the efficiency of vaccine preparation. Glycosylated MUC1 tripartite vaccines were constructed through host–guest interactions with cucurbit[8]uril. These vaccines elicited high levels of IgG antibodies that were recognized by transformed cells and induced the secretion of cytokines. The antisera also mediated complement‐dependent cytotoxicity. This noncovalent strategy with good suitability, scalability, and feasibility can be applied as a universal strategy for the construction of chemically synthesized vaccines.