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Enhanced Iridium Complex Electrochemiluminescence Cytosensing and Dynamic Evaluation of Cell‐Surface Carbohydrate Expression
Author(s) -
Zhou Hong,
Yang Yiying,
Li Chunxiang,
Yu Bing,
Zhang Shusheng
Publication year - 2014
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201403470
Subject(s) - electrochemiluminescence , nanoprobe , detection limit , nanoparticle , nanomaterials , mesoporous silica , electrode , materials science , chemistry , graphene , colloidal gold , nanotechnology , mesoporous material , chromatography , biochemistry , catalysis
A newly prepared [(ppy) 2 Ir(dcbpy)] + ⋅PF 6 − (ppy: 2‐phenylpyridyl; dcbpy: 4,4′‐dicarboxy‐2,2′‐bipyridyl) and gold nanoparticle functionalized mesoporous silica nanoparticle (Au/Ir‐MSN) is reported. Based on the binding between concanavalin A (Con A) and mannose, the novel nanoparticle was applied to an ultrasensitive electrochemiluminescence (ECL) in situ cytosensing strategy and the dynamic evaluation of cell‐surface carbohydrate expression. The ECL activity of the presented Con A@Au/Ir‐MSN nanoprobe was greatly enhanced by employing a functionalized nanoparticle and graphene nanomaterial with an increased surface area and simultaneously improved electron‐transfer efficiency at the electrode interface. Under optimal conditions, the sandwich‐type ECL cytosensor showed a linear response to K562 cells at concentrations ranging from 1.0×10 2 to 1.0×10 6 cells mL −1 and realized a low detection limit of a single cell. The proposed method could also be successfully used for monitoring the dynamic variation of carbohydrate expression in cancer cells in response to external stimulation by an inhibitor.