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Directly Decoupled Diffusion‐Ordered NMR Spectroscopy for the Analysis of Compound Mixtures
Author(s) -
Glanzer Simon,
Zangger Klaus
Publication year - 2014
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201402920
Subject(s) - homonuclear molecule , decoupling (probability) , chemistry , spectroscopy , nuclear magnetic resonance spectroscopy , analytical chemistry (journal) , diffusion , spectral line , two dimensional nuclear magnetic resonance spectroscopy , chromatography , molecule , organic chemistry , stereochemistry , physics , thermodynamics , quantum mechanics , control engineering , astronomy , engineering
For the analysis of compound mixtures by NMR spectroscopy, it is important to assign the different peaks to the individual constituents. Diffusion‐ordered spectroscopy (DOSY) is often used for the separation of signals based on their self‐diffusion coefficient. However, this method often fails in the case of signal overlap, which is a particular problem for 1 H‐detected DOSY spectra. Herein, an approach that allows the acquisition of homonuclear broadband‐decoupled DOSY spectra without the introduction of an additional decoupling dimension, by instant decoupling during acquisition, is presented. It was demonstrated on a mixture of six alcohols, and the investigation of the binding of a dodecapeptide to membrane mimetics.