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Hybrid Mesoporous Silica Nanoparticles with pH‐Operated and Complementary H‐Bonding Caps as an Autonomous Drug‐Delivery System
Author(s) -
Théron Christophe,
Gallud Audrey,
Carcel Carole,
GaryBobo Magali,
Maynadier Marie,
Garcia Marcel,
Lu Jie,
Tamanoi Fuyuhiko,
Zink Jeffrey I.,
Wong Chi Man Michel
Publication year - 2014
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201402864
Subject(s) - mesoporous silica , camptothecin , rhodamine b , propidium iodide , drug delivery , chemistry , nanoparticle , biophysics , cytotoxicity , nuclear chemistry , nanotechnology , materials science , mesoporous material , programmed cell death , in vitro , organic chemistry , biochemistry , apoptosis , photocatalysis , biology , catalysis
Mesoporous silica nanoparticles (MSNPs) are functionalized with molecular‐recognition sites by anchoring a triazine or uracil fragment on the surface. After loading these MSNPs with dyes (propidium iodide or rhodamine B) or with a drug (camptothecin, CPT) they are capped by the complementary fragments, uracil and adenine, respectively, linked to the bulky cyclodextrin ring. These MSNPs are pH‐sensitive and indeed, the dye release was observed at acidic pH by continuously monitored fluorescence spectroscopy studies. On the other hand, no dye leakage occurred at neutral pH, hence meeting the non‐premature requirement to minimize side effects. In vitro studies were performed and confocal microscopy images demonstrate the internalization of the MSNPs and also dye release in the cells. To investigate the drug‐delivery performance, the cytotoxicity of CPT‐loaded nanoparticles was tested and cell death was observed. A remarkably lower amount of loaded CPT in the MSNPs (more than 40 times less) proved to be as efficient as free CPT. These results not only demonstrate the drug release after pore opening under lysosomal pH, but they also show the potential use of these MSNPs to significantly decrease the amount of the administered drug.