Design of Maleimide‐Functionalised Electrodes for Covalent Attachment of Proteins through Free Surface Cysteine Groups | Zendy

Wright Emma J. | Zendy; Sosna Maciej | Zendy; Bloodworth Sally | Zendy; Kilburn Jeremy D. | Zendy; Bartlett Philip N. | Zendy

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Design of Maleimide‐Functionalised Electrodes for Covalent Attachment of Proteins through Free Surface Cysteine Groups

Author(s) -

Wright Emma J.,

Sosna Maciej,

Bloodworth Sally,

Kilburn Jeremy D.,

Bartlett Philip N.

Publication year - 2014

Publication title -

chemistry – a european journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.687

H-Index - 242

eISSN - 1521-3765

pISSN - 0947-6539

DOI - 10.1002/chem.201400246

Subject(s) - maleimide , covalent bond , chemistry , acetamide , amide , cysteine , redox , polymer chemistry , monolayer , hexamethylenediamine , electrochemistry , electrode , thiol , self assembled monolayer , combinatorial chemistry , organic chemistry , polyamide , enzyme , biochemistry

Mixed two‐component monolayers on glassy carbon are prepared by electrochemical oxidation of N ‐(2‐aminoethyl)acetamide and mono‐ N ‐Boc‐hexamethylenediamine in mixed solution. Subsequent N ‐deprotection, amide coupling and solid‐phase synthetic steps lead to electrode‐surface functionalisation with maleimide, with controlled partial coverage of this cysteine‐binding group at appropriate dilution for covalent immobilisation of a model redox‐active protein, cytochrome c , with high coverage (≈7.5 pmol cm −2 ).

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