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Back Cover: Rational Design for Cooperative Recognition of Specific Nucleobases Using β‐Cyclodextrin‐Modified DNAs and Fluorescent Ligands on DNA and RNA Scaffolds (Chem. Eur. J. 32/2013)
Author(s) -
Futamura Akika,
Uemura Asuka,
Imoto Takeshi,
Kitamura Yusuke,
Matsuura Hirotaka,
Wang ChunXia,
Ichihashi Toshiki,
Sato Yusuke,
Teramae Norio,
Nishizawa Seiichi,
Ihara Toshihiro
Publication year - 2013
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201390120
Subject(s) - nucleobase , oligonucleotide , dna , chemistry , fluorophore , conjugate , fluorescence , combinatorial chemistry , cyclodextrin , rna , ligand (biochemistry) , moiety , stereochemistry , biophysics , biochemistry , biology , receptor , mathematical analysis , physics , mathematics , quantum mechanics , gene
A binary fluorimetric method for DNA and RNA analysis is proposed based on the combination of two probes designed to work cooperatively. One is an oligonucleotide conjugate bearing a β‐cyclodextrin. The other is a small reporter ligand, which comprises linked molecules of a nucleobase‐specific heterocycle and an environment‐sensitive fluorophore. The reporter ligand recognizes a single nucleobase displayed in a gap on the target labeled with the conjugate, and the fluorophore moiety forms a luminous inclusion complex with nearby cyclodextrin. For more details see the Full Paper by S. Nishizawa, T. Ihara et al. on page 10526 ff.