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Sensing of Phosphates by Using Luminescent Eu III and Tb III Complexes: Application to the Microalgal Cell Chlorella vulgaris
Author(s) -
Nadella Sandeep,
Sahoo Jashobanta,
Subramanian Palani S.,
Sahu Abhishek,
Mishra Sandhya,
Albrecht Markus
Publication year - 2014
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201304664
Subject(s) - luminescence , europium , chemistry , fluorescence , terbium , nucleoside , quenching (fluorescence) , cell wall , nuclear chemistry , adenosine , adenosine monophosphate , polyphosphate , crystallography , stereochemistry , biophysics , biochemistry , phosphate , organic chemistry , materials science , ion , biology , physics , optoelectronics , quantum mechanics
Phenanthroline‐based chiral ligands L 1 and L 2 as well as the corresponding Eu III and Tb III complexes were synthesized and characterized. The coordination compounds show red and green emission, which was explored for the sensing of a series of anions such as F − , Cl − , Br − , I − , NO 3 − , NO 2 − , HPO 4 2− , HSO 4 − , CH 3 COO − , and HCO 3 − . Among the anions, HPO 4 2− exhibited a strong response in the emission property of both europium(III) and terbium(III) complexes. The complexes showed interactions with the nucleoside phosphates adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP). Owing to this recognition, these complexes have been applied as staining agents in the microalgal cell Chlorella vulgaris . The stained microalgal cells were monitored through fluorescence microscopy and scanning electron microscopy. Initially, the complexes bind to the outer cell wall and then enter the cell wall through holes in which they probably bind to phospholipids. This leads to a quenching of the luminescence properties.