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Aptamer‐Based Luminescence Energy Transfer from Near‐Infrared‐to‐Near‐Infrared Upconverting Nanoparticles to Gold Nanorods and Its Application for the Detection of Thrombin
Author(s) -
Yuan Fei,
Chen Hongqi,
Xu Juan,
Zhang Yiyan,
Wu Yong,
Wang Lun
Publication year - 2014
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201304556
Subject(s) - thrombin , aptamer , nanorod , photon upconversion , luminescence , nanoparticle , near infrared spectroscopy , materials science , acceptor , analytical chemistry (journal) , photochemistry , chemistry , nanotechnology , optoelectronics , chromatography , optics , platelet , genetics , physics , condensed matter physics , immunology , biology
A new luminescence energy transfer (LET) system has been designed for the detection of thrombin in the near‐infrared (NIR) region by utilizing NIR‐to‐NIR upconversion lanthanide nanophosphors (UCNPs) as the donor and gold nanorods (Au NRs) as the acceptor. The use of upconverting NaYF 4 :Yb 3+ ,Tm 3+ nanoparticles with sharp NIR emission peaks upon NIR excitation by an inexpensive infrared continuous wave laser diode provided large spectral overlap between the donor and the acceptor. Both the Au NRs and carboxyl‐terminated NaYF 4 :Yb 3+ ,Tm 3+ UCNPs were first modified with different thrombin aptamers. When thrombin was added, a LET system was then formed because of the specific recognition between the thrombin aptamers and thrombin. The LET system was used to monitor thrombin concentrations in aqueous buffer and human blood samples. The limits of detection for thrombin are as low as 0.118 n M in buffer solution and 0.129 n M in human serum. The method was also successfully applied to thrombin detection in blood samples.