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Filling Carbon Nanotubes with Prussian Blue Nanoparticles of High Peroxidase‐Like Catalytic Activity for Colorimetric Chemo‐ and Biosensing
Author(s) -
Wang Ting,
Fu Yingchun,
Chai Liyuan,
Chao Long,
Bu Lijuan,
Meng Yue,
Chen Chao,
Ma Ming,
Xie Qingji,
Yao Shouzhuo
Publication year - 2014
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201304035
Subject(s) - prussian blue , glucose oxidase , carbon nanotube , nanoparticle , detection limit , biosensor , absorbance , catalysis , nanocomposite , peroxidase , chemical engineering , materials science , chemistry , nanotechnology , nuclear chemistry , chromatography , electrochemistry , organic chemistry , electrode , enzyme , engineering
Facile filling of multiwalled carbon nanotubes (MWCNTs) with Prussian blue nanoparticles (PBNPs) of high peroxidase‐like catalytic activity was performed to develop novel colorimetric sensing protocols for assaying H 2 O 2 and glucose. Fine control of PBNP growth was achieved by modulating the concentration ratio of K 3 [Fe(CN) 6 ] and FeSO 4 precursors in an acidic solution containing ultrasonically dispersed MWCNTs, and thus size‐matched PBNPs could be robustly immobilized in the cavities of the MWCNTs (MWCNT‐PB in ). Unlike other reported methods involving complicated procedures and rigorous preparation/separation conditions, this mild one‐pot filling method has advantages of easy isolation of final products by centrifugation, good retention of the pristine outer surface of the MWCNT shell, and satisfactory filling yield of (24±2) %. In particular, encapsulation of PBNPs of poor dispersibility and limited functionality in dispersible and multifunctional MWCNT shells creates new and valuable opportunities for quasihomogeneous‐phase applications of PB in liquid solutions. The MWCNT‐PB in nanocomposites were exploited as a peroxidase mimic for the colorimetric assay of H 2 O 2 in solution by using 3,3′,5,5′‐tetramethylbenzidine (TMB) as reporter, and they gave a linear absorbance response from 1 μ M to 1.5 m M with a limit of detection (LOD) of 100 n M . Moreover, glucose oxidase (GOx) was anchored on the outer surface of MWCNT‐PB in to form GOx/MWCNT‐PB in bionanocomposites. The cooperation of outer‐surface biocatalysis with peroxidase‐like catalysis of interior PB resulted in a novel cooperative colorimetric biosensing mode for glucose assay. The use of GOx/MWCNT‐PB in for colorimetric biosensing of glucose gave a linear absorbance response from 1 μ M to 1.0 m M and an LOD of 200 n M . The presented protocols may be extended to other multifunctional nanocomposite systems for broad applications in catalysis and biotechnology.