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Directed Evolution of 2G12‐Targeted Nonamannose Glycoclusters by SELMA
Author(s) -
Temme J. Sebastian,
Drzyzga Michael G.,
MacPherson Iain S.,
Krauss Isaac J.
Publication year - 2013
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201303848
Subject(s) - glycan , computational biology , human immunodeficiency virus (hiv) , cluster analysis , computer science , biology , genetics , virology , glycoprotein , artificial intelligence
Optimizing multivalency : Clusters of Man 9 glycans, which are recognized by broadly neutralizing anti‐HIV antibody 2G12, have potential as HIV vaccines. However, optimal recognition by 2G12 requires optimum clustering of glycans. Using the recently described SELMA technique (SELection with Modified Aptamers), Man 9 clusters have been developed, in which glycans are supported by DNA sequences selected from among 2×10 13 variants (see scheme).