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Three‐Pronged Probes: High‐Affinity DNA Binding with Cap, β‐Alanines and Oligopyrrolamides
Author(s) -
Haug Rüdiger,
Kramer Markus,
Richert Clemens
Publication year - 2013
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201302972
Subject(s) - stacking , oligonucleotide , dna , minor groove , base pair , biophysics , chemistry , point mutation , groove (engineering) , microbiology and biotechnology , stereochemistry , crystallography , mutant , biochemistry , biology , materials science , gene , organic chemistry , metallurgy
TPP oligonucleotides : Hybridization probes that interrogate target sequences through base pairing, stacking on the terminus, and binding in the minor groove are presented (see figure). All subunits of the probes contribute to the target affinity, leading to melting point increases of up to 45 °C. (TPP=three‐pronged probe)