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Fluorescent Protein Capped Mesoporous Nanoparticles for Intracellular Drug Delivery and Imaging
Author(s) -
Yang Xinjian,
Li Zhenhua,
Li Meng,
Ren Jinsong,
Qu Xiaogang
Publication year - 2013
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201302026
Subject(s) - drug delivery , nanotechnology , mesoporous silica , nanoparticle , fluorescence , nanopore , materials science , intracellular , biophysics , mesoporous material , nanobiotechnology , fluorescence lifetime imaging microscopy , chemistry , biochemistry , physics , quantum mechanics , biology , catalysis
A multifunctional system for intracellular drug delivery and simultaneous fluorescent imaging was constructed by using histidine‐tagged, cyan fluorescent protein (CFP)‐capped magnetic mesoporous silica nanoparticles (MMSNs). This protein‐capped multifunctional nanostructure is highly biocompatible and does not affect cell viability or proliferation. The CFP acts not only as a capping agent, but also as a fluorescent imaging agent. The nanoassembly was activated by histidine‐based replacement, leading to release of drug molecules encapsulated in the nanopores into the bulk solution. The fluorescent imaging functionality would allow noninvasive tracking of the nanoparticles in the body. By combining the drug delivery with cell‐imaging capability, these nanoparticles may provide valuable multifunctional nanoplatforms for biomedical applications.