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Bi‐Enzyme Sensor for Phenolic Compounds with Fluorescent Read‐Out
Author(s) -
Strianese Maria,
Zauner Gerhild,
Tabares Leandro C,
Tepper Armand W. J. W.,
De Martino Franco,
Pellecchia Claudio,
Aartsma Thijs J.,
Canters Gerard W.
Publication year - 2013
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201301876
Subject(s) - fluorometer , fluorescence , phenols , chemistry , tyrosinase , phenol , enzyme , chromatography , biochemistry , trace (psycholinguistics) , trace amounts , organic chemistry , medicine , physics , linguistics , philosophy , alternative medicine , pathology , quantum mechanics
In this paper, the use of tyrosinase (Ty) from Streptomyces antibioticus , labeled with a fluorescent tag, in combination with soluble quinoprotein (PQQ‐containing) glucose dehydrogenase ( s‐ GDH) to measure trace amounts of phenols is explored. Proof of concept is provided by a series of experiments, which show a clear quantitative dependence of the response on the phenol concentration. One of the advantages of the detection system is that apart from a standard fluorimeter no further instrumentation is required.