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Selective Chemical Labelling of 5‐Formylcytosine in DNA by Fluorescent Dyes
Author(s) -
Hu Jianlin,
Xing Xiwen,
Xu Xiaowei,
Wu Fan,
Guo Pu,
Yan Shengyong,
Xu Zhenghao,
Xu Jiahui,
Weng Xiaocheng,
Zhou Xiang
Publication year - 2013
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201300082
Subject(s) - labelling , dna , fluorescence , cytosine , computer science , chemistry , information retrieval , biochemistry , physics , quantum mechanics
Direct labelling : 5‐Formylcytosine in DNA can be selectively labelled by fluorescent dyes containing an active amino group. The labelled DNA shows strong fluorescence and can be detected by polyacrylamide gel electrophoresis (PAGE) and fluorescence measurements (see scheme). This method can distinguish 5‐formylcytosine from other methylation forms of cytosine in DNA.