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Enzyme‐Responsive Silica Mesoporous Supports Capped with Azopyridinium Salts for Controlled Delivery Applications
Author(s) -
Mas Núria,
Agostini Alessandro,
Mondragón Laura,
Bernardos Andrea,
Sancenón Félix,
Marcos M. Dolores,
MartínezMáñez Ramón,
Costero Ana M.,
Gil Salvador,
MerinoSanjuán Matilde,
Amorós Pedro,
Orzáez Mar,
PérezPayá Enrique
Publication year - 2013
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201202740
Subject(s) - mesoporous silica , hela , rhodamine b , camptothecin , internalization , rhodamine , nanoparticle , drug delivery , cytotoxicity , chemistry , controlled release , nanotechnology , materials science , mesoporous material , biophysics , in vitro , organic chemistry , cell , biochemistry , photocatalysis , fluorescence , physics , quantum mechanics , biology , catalysis
The preparation of a new capped silica mesoporous material, Rh‐Azo‐S , for on‐command delivery applications in the presence of target enzymes is described. The material consists of nanometric mesoporous MCM‐41‐like supports loaded with Rhodamine B and capped with an azopyridine derivative. The material was designed to show “zero delivery” and to display a cargo release in the presence of reductases and esterases, which are usually present in the colon, mainly due to intestinal microflora. The opening and cargo release of Rh‐Azo‐S in vitro studies were assessed and seen to occur in the presence of these enzymes, whereas no delivery was noted in the presence of pepsine. Moreover, Rh‐Azo‐S nanoparticles were used to study controlled Rhodamine B dye delivery in intracellular media. HeLa cells were employed for testing the “non”‐toxicity of nanoparticles. Moreover, delivery of the dye in these cells, through internalization and enzyme‐mediated gate opening, was confirmed by confocal microscopy. Furthermore, the nanoparticles capped with the Azo group and loaded with a cytotoxic camptothecin ( CPT ) were also prepared (solid CPT‐Azo‐S ) and used as delivery nanodevices in HeLa cells. When this solid was employed, the cell viability decreased significantly due to internalization of the nanoparticles and delivery of the cytotoxic agent.