Premium
Rapid and Specific Post‐Synthesis Modification of DNA through a Biocompatible Condensation of 1,2‐Aminothiols with 2‐Cyanobenzothiazole
Author(s) -
Cheng Yunfeng,
Peng Hanjing,
Chen Weixuan,
Ni Nanting,
Ke Bowen,
Dai Chaofeng,
Wang Binghe
Publication year - 2013
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201201677
Subject(s) - surface modification , chemistry , combinatorial chemistry , dna , condensation , yield (engineering) , dna synthesis , materials science , biochemistry , physics , metallurgy , thermodynamics
Post‐synthesis modification of DNA is an important way of functionalizing DNA molecules. Herein, we describe a method that first enzymatically incorporates a cyanobenzothiazole (CBT)‐modified thymidine. The side‐chain handle CBT can undergo a rapid and site‐specific cyclization reaction with 1,2‐aminothiols to afford DNA functionalization in aqueous solution. Another key advantage of this method is the formation of a single stereo/regioisomer in the process, which allows for precise control of DNA modification to yield a single component for aptamer selection work and other applications.