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The Vital Role of Buffer Anions in the Antioxidant Activity of CeO 2 Nanoparticles
Author(s) -
Xue Ying,
Zhai Yanwu,
Zhou Kebin,
Wang Lei,
Tan Haina,
Luan Qingfen,
Yao Xin
Publication year - 2012
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201200983
Subject(s) - nanoparticle , antioxidant , cerium oxide , chemistry , cerium , phosphate , reactive oxygen species , tris , phosphate buffered saline , inorganic chemistry , buffer (optical fiber) , oxide , nanotechnology , materials science , organic chemistry , biochemistry , chromatography , telecommunications , computer science
Cerium oxide (CeO 2 ) nanoparticles display excellent antioxidant properties by scavenging free radicals. However, some studies have indicated that they can cause an adverse response by generating reactive oxygen species (ROS). Hence, it is important to clarify the factors that affect the oxidant/antioxidant activities of CeO 2 nanoparticles. In this work, we report the effects of different buffer anions on the antioxidant activity of CeO 2 nanoparticles. Considering the main anions present in the body, Tris‐HCl, sulfate, and phosphate buffer solutions have been used to evaluate the antioxidant activity of CeO 2 nanoparticles by studying their DNA protective effect. The results show that CeO 2 nanoparticles can protect DNA from damage in Tris‐HCl and sulfate systems, but not in phosphate buffer solution (PBS) systems. The mechanism of action has been explored: cerium phosphate is formed on the surface of the nanoparticles, which interferes with the redox cycling between Ce 3+ and Ce 4+ . As a result, the antioxidant activity of CeO 2 nanoparticles is greatly affected by the external environment, especially the anions. These results may provide guidance for the further practical application of CeO 2 nanoparticles.