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A Way to Probe the Microenvironment of Free Sulfhydryls in Intact Proteins with a Series of Monofunctional Organic Mercurials
Author(s) -
Xu Ming,
Yang Limin,
Wang Qiuquan
Publication year - 2012
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201200901
Subject(s) - chemistry , series (stratigraphy) , biochemistry , biology , paleontology
Monofunctional organic mercurials (MFOHgs) of different sizes and electronic properties were used to evaluate the accessibility of SH groups in intact proteins. The change in conformation of proteins, such as BLG and OVA, when treated with MFOHgs of increasing size was used as a measure of the accessibility of SH groups contained within these proteins (see picture).