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Ratiometric Fluorescent Signaling of Small Molecule, Environmentally Sensitive Dye Conjugates for Detecting Single‐Base Mutations in DNA
Author(s) -
Wang Chunxia,
Sato Yusuke,
Kudo Megumi,
Nishizawa Seiichi,
Teramae Norio
Publication year - 2012
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201200219
Subject(s) - conjugate , nucleobase , dna , chemistry , combinatorial chemistry , fluorescence , pyrimidine , förster resonance energy transfer , ligand (biochemistry) , computational biology , biochemistry , biology , receptor , mathematical analysis , physics , mathematics , quantum mechanics
Bind it! A ratiometric fluorescent probe based on conjugation of a DNA‐binding ligand with an environmentally sensitive dye to probe hydrophobic grooves of the DNA helix was developed. The resulting conjugate ATMND–DBD can bind to an orphan nucleobase opposite an abasic (AP) site with high selectivity and affinity for pyrimidine bases (see figure).