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Inside Cover: A 5′‐Cap for DNA Probes Binding RNA Target Strands (Chem. Eur. J. 42/2011)
Author(s) -
Egetenmeyer Simone,
Richert Clemens
Publication year - 2011
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201190206
Subject(s) - oligonucleotide , base pair , dna , rna , chemistry , covalent bond , cover (algebra) , helix (gastropod) , biophysics , selectivity , biochemistry , biology , gene , engineering , mechanical engineering , ecology , organic chemistry , snail , catalysis
To cap or not to cap? In their Full Paper on page 11813 ff. , S. Egetenmeyer and C. Richert describe a covalently attached cap for the 5′‐terminus of oligonucleotides that increases base pairing fidelity by binding tightly to the helix when a canonical base pair is formed, but providing little stabilization in the case of a mismatch. The resulting enhancement in affinity and selectivity aids the detection of microRNAs.