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Cover Picture: Targeted Cellular Uptake and siRNA Silencing by Quantum‐Dot Nanoparticles Coated with β‐Cyclodextrin Coupled to Amino Acids (Chem. Eur. J. 18/2011)
Author(s) -
Zhao MeiXia,
Li JinMing,
Du Lingyan,
Tan CaiPing,
Xia Qing,
Mao ZongWan,
Ji LiangNian
Publication year - 2011
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201190087
Subject(s) - endocytosis , gene silencing , hela , intracellular , fluorescence , quantum dot , cyclodextrin , biophysics , cytoplasm , chemistry , conjugated system , amino acid , cell , biochemistry , nanotechnology , materials science , gene , biology , physics , polymer , organic chemistry , quantum mechanics
siRNA was delivered by quantum dots (QDs) modified with functional molecules to silence targeted genes in cells. FITC‐labeled siRNA was conjugated with QDs coated with β‐cyclodextrin (β‐CD) coupled to amino acids, and resulted in a decrease in the fluorescence intensity. In addition, siRNA was delivered into the cytoplasm of HeLa cells by endocytosis and then released to specifically silence the HPV18 E6 gene under action of the RISC enzyme. These QDs provide a bright and stable fluorescent signal for intracellular siRNA imaging. For more details see the Full Paper by Z.‐W. Mao, Q. Xia et al. on page 5171 ff.