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A Cationic Phospholipid–Detergent Conjugate as a New Efficient Carrier for siRNA Delivery
Author(s) -
Pierrat Philippe,
Creusat Gaëlle,
Laverny Gilles,
Pons Françoise,
Zuber Guy,
Lebeau Luc
Publication year - 2012
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201103645
Subject(s) - conjugate , cationic polymerization , phospholipid , cationic liposome , chemistry , nucleic acid , moiety , liposome , gene silencing , biochemistry , combinatorial chemistry , membrane , stereochemistry , transfection , gene , organic chemistry , mathematical analysis , mathematics
A “grapnel” lipid : Conjugation of Triton X‐100 with DOPC provides a new class of nucleic acid carriers (see figure). The lamellar phase forming a cationic conjugate has no hemolytic activity and efficiently delivers siRNA into the cytosol of mammalian cells. Covalent anchoring of the detergent to the phospholipid and the dangling hydrophobic part of the detergent moiety appear to be essential for siRNA delivery and efficient gene silencing.