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Boronic Acid Functionalized Core–Shell Polymer Nanoparticles Prepared by Distillation Precipitation Polymerization for Glycopeptide Enrichment
Author(s) -
Qu Yanyan,
Liu Jianxi,
Yang Kaiguang,
Liang Zhen,
Zhang Lihua,
Zhang Yukui
Publication year - 2012
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201103514
Subject(s) - polymerization , distillation , precipitation , glycopeptide , polymer , precipitation polymerization , core (optical fiber) , nanoparticle , chemical engineering , boronic acid , chemistry , materials science , polymer chemistry , organic chemistry , nanotechnology , radical polymerization , composite material , biochemistry , antibiotics , physics , meteorology , engineering
The boronic acid‐functionalized core–shell polymer nanoparticles, poly( N , N ‐methylenebisacrylamide‐co‐methacrylic acid)@4‐vinylphenylboronic acid (poly(MBA‐co‐MAA)@VPBA), were successfully synthesized for enriching glycosylated peptides. Such nanoparticles were composed of a hydrophilic polymer core prepared by distillation precipitation polymerization (DPP) and a boronic acid‐functionalized shell designed for capturing glycopeptides. Owing to the relatively large amount of residual vinyl groups introduced by DPP on the core surface, the VPBA monomer was coated with high efficiency, working as the shell. Moreover, the overall polymerization route, especially the use of DPP, made the synthesis of nanoparticles facile and time‐saving. With the poly(MBA‐co‐MAA)@VPBA nanoparticles, 18 glycopeptides from horseradish peroxidase (HRP) digest were captured and identified by MALDI‐TOF mass spectrometric analysis, relative to eight glycopeptides enriched by using commercially available meta ‐aminophenylboronic acid agarose under the same conditions. When the concentration of the HRP digest was decreased to as low as 5 nmol, glycopeptides could still be selectively isolated by the prepared nanoparticles. Our results demonstrated that the synthetic poly(MBA‐co‐MAA)@VPBA nanoparticles might be a promising selective enrichment material for glycoproteome analysis.