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Determination of the Absolute Configuration of Peptide Natural Products by Using Stable Isotope Labeling and Mass Spectrometry
Author(s) -
Bode Helge B.,
Reimer Daniela,
Fuchs Sebastian W.,
Kirchner Ferdinand,
Dauth Christina,
Kegler Carsten,
Lorenzen Wolfram,
Brachmann Alexander O.,
Grün Peter
Publication year - 2012
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201103479
Subject(s) - absolute configuration , chemistry , mass spectrometry , isotope , isolation (microbiology) , chromatography , stereochemistry , biology , bioinformatics , physics , quantum mechanics
Structure elucidation of natural products including the absolute configuration is a complex task that involves different analytical methods like mass spectrometry, NMR spectroscopy, and chemical derivation, which are usually performed after the isolation of the compound of interest. Here, a combination of stable isotope labeling of Photorhabdus and Xenorhabdus strains and their transaminase mutants followed by detailed MS analysis enabled the structure elucidation of novel cyclopeptides named GameXPeptides including their absolute configuration in crude extracts without their actual isolation.