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Monodisperse REPO 4 (RE=Yb, Gd, Y) Hollow Microspheres Covered with Nanothorns as Affinity Probes for Selectively Capturing and Labeling Phosphopeptides
Author(s) -
Cheng Gong,
Zhang JiLin,
Liu YanLin,
Sun DeHui,
Ni JiaZuan
Publication year - 2012
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201103328
Subject(s) - dispersity , scanning electron microscope , fourier transform infrared spectroscopy , transmission electron microscopy , adsorption , materials science , phosphate , analytical chemistry (journal) , chemistry , nuclear chemistry , chromatography , chemical engineering , nanotechnology , polymer chemistry , organic chemistry , engineering , composite material
Rare‐earth phosphate microspheres with unique structures were developed as affinity probes for the selective capture and tagging of phosphopeptides. Prickly REPO 4 (RE=Yb, Gd, Y) monodisperse microspheres, that have hollow structures, low densities, high specific surface areas, and large adsorptive capacities were prepared by an ion‐exchange method. The elemental compositions and crystal structures of these affinity probes were confirmed by energy‐dispersive spectroscopy (EDS), powder X‐ray diffraction (XRD), and Fourier‐transform infrared (FTIR) spectroscopy. The morphologies of these compounds were investigated using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and nitrogen‐adsorption isotherms. The potential ability of these microspheres for selectively capturing and labeling target biological molecules was evaluated by using protein‐digestion analysis and a real sample as well as by comparison with the widely used TiO 2 affinity microspheres. These results show that these porous rare‐earth phosphate microspheres are highly promising probes for the rapid purification and recognition of phosphopeptides.