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Rate‐Controlling Isomerizations in Fatty Acid Oxidations by a Cytochrome P450 Compound I
Author(s) -
Su Zhi,
Chen Xiaohong,
Horner John H.,
Newcomb Martin
Publication year - 2012
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201103170
Subject(s) - chemistry , isomerization , lauric acid , fatty acid , kinetic isotope effect , cytochrome p450 , hydroxylation , stereochemistry , cytochrome , photochemistry , metabolism , organic chemistry , biochemistry , deuterium , catalysis , enzyme , physics , quantum mechanics
KIE events : Hydroxylations of fatty acids by cytochrome P450 119 Compound I display no intermolecular kinetic isotope effect (KIE) in buffer and increase in rate with increasing chain length (see graph). With glycerol, the rate of reaction of lauric acid increases, and the KIE is revealed. The mechanism involves reversible formation of an unreactive complex of a fatty acid with Compound I and its isomerization to a reactive one.