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The Alzheimer’s Disease Related Tau Protein as a New Target for Chemical Protein Engineering
Author(s) -
Broncel Malgorzata,
Krause Eberhard,
Schwarzer Dirk,
Hackenberger Christian P. R.
Publication year - 2012
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201103032
Subject(s) - biotinylation , phosphorylation , computer science , tau protein , computational biology , information retrieval , artificial intelligence , alzheimer's disease , chemistry , biochemistry , biology , disease , medicine , pathology
Site‐specifically phosphorylated tau : A semisynthetic strategy has been applied for the first time to generate a phosphorylated and biotinylated fully functional tau protein (see figure). The presented methodology allows for an unambiguous verification of individual phosphorylation sites on tau and significantly improves its purification.