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Calcium Carbonate–Gold Nanocluster Hybrid Spheres: Synthesis and Versatile Application in Immunoassays
Author(s) -
Peng Juan,
Feng LiNa,
Zhang Kui,
Li XingHua,
Jiang LiPing,
Zhu JunJie
Publication year - 2012
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201102876
Subject(s) - nanoclusters , horseradish peroxidase , biocompatibility , fluorescence , chemistry , detection limit , nanoparticle , electrochemistry , solubility , nanotechnology , materials science , chemical engineering , chromatography , organic chemistry , electrode , physics , quantum mechanics , engineering , enzyme
Fluorescent gold nanoclusters (AuNCs) were incorporated into porous calcium carbonate spheres through electrostatic interaction. The resulting CaCO 3 /AuNCs hybrid material exhibited interesting properties, such as porous structure, excellent biocompatibility, good water solubility, and degradability. These properties make the CaCO 3 /AuNCs hybrid material a promising template to assemble horseradish peroxidase/antibody conjugates (HRP‐Ab 2 ). By using CaCO 3 /AuNCs/HRP‐Ab 2 bioconjugates as probes, a versatile immunosensor was developed for fluorescent and electrochemical detection of the cancer biomarker neuron‐specific enolase (NSE). The detection limits of the sensor were 2.0 and 0.1 pg mL −1 for fluorescent and electrochemical detection, respectively. The immunosensor shows high sensitivity and offers an alternative strategy for the detection of other proteins and DNA.