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DNA–Polymer Micelles as Nanoparticles with Recognition Ability
Author(s) -
Talom Renée Mayap,
Fuks Gad,
Kaps Leonard,
Oberdisse Julian,
Cerclier Christel,
Gaillard Cédric,
Mingotaud Christophe,
Gauffre Fabienne
Publication year - 2011
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201101561
Subject(s) - micelle , quartz crystal microbalance , polymer , copolymer , materials science , nanoparticle , dynamic light scattering , chemical engineering , dna , nanotechnology , chemistry , organic chemistry , adsorption , composite material , aqueous solution , engineering , biochemistry
The Watson–Crick binding of DNA single strands is a powerful tool for the assembly of nanostructures. Our objective is to develop polymer nanoparticles equipped with DNA strands for surface‐patterning applications, taking advantage of the DNA technology, in particular, recognition and reversibility. A hybrid DNA copolymer is synthesized through the conjugation of a ssDNA (22‐mer) with a poly(ethylene oxide)‐poly(caprolactone) diblock copolymer (PEO‐ b ‐PCl). It is shown that, in water, the PEO‐ b ‐PCl‐ssDNA 22 polymer forms micelles with a PCl hydrophobic core and a hydrophilic corona made of PEO and DNA. The micelles are thoroughly characterized using electron microscopy (TEM and cryoTEM) and small‐angle neutron scattering. The binding of these DNA micelles to a surface through DNA recognition is monitored using a quartz crystal microbalance and imaged by atomic force microscopy. The micelles can be released from the surface by a competitive displacement event.